DNA

Part:BBa_K315026:Design

Designed by: Nitya Rao   Group: iGEM10_Davidson-MissouriW   (2010-07-15)


pTet + lox5171 forward + RBS + RFP + loxN forward


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 677
    Illegal AgeI site found at 789
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pTet is a moderately strong promoter. RFP is a readily visible reporter.


Source

We created this part in an effort to determine how various lox sites interact.

References